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化工儀器網>產品展廳>試劑標物>行業專用試劑>生物試劑> 甲狀腺過氧化物酶抗體

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甲狀腺過氧化物酶抗體

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產品編號bs-10406R
英文名稱Thyroid peroxidase
中文名稱甲狀腺過氧化物酶抗體
別    名MSA; PERT_HUMAN; TDH2A; Thyroid microsomal antigen; Thyroid peroxidase; Thyroperoxidase; TPO; TPX.  
規格價格0.1ml/780元 購買    0.2ml/1280元 購買    
說 明 書0.1ml  0.2ml  
研究領域
抗體來源Rabbit
克隆類型Polyclonal
交叉反應 Human, Mouse, Rat, 
產品應用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量101kDa
細胞定位細胞核 
性    狀Lyophilized or Liquid
濃    度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Thyroid peroxidase
亞    型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
生 產 商BIOSS
PubMedPubMed
甲狀腺過氧化物酶抗體產品介紹background:
This gene encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, May 2011].

Function:
Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).

Subunit:
Interacts with DUOX1, DUOX2 and CYBA.

Subcellular Location:
Membrane; Single-pass type I membrane protein. 
Isoform 3: Cell surface.

Post-translational modifications:
Glycosylated. 
Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface. 
Cleaved in its N-terminal part.

DISEASE:
Note=An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease. 
Defects in TPO are the cause of thyroid dyshormonogenesis 2A (TDH2A) [MIM:274500]. A disorder due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete.

Similarity:
Belongs to the peroxidase family. XPO subfamily.
Contains 1 EGF-like domain.
Contains 1 Sushi (CCP/SCR) domain.

SWISS:
P07202

Database links:
NCBI Reference Sequence: NP_000538.3
UniProtKB/Swiss-Prot: P07202.4

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產品圖片
Tissue/cell: Rat thyroid; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Anti-Thyroid peroxidase Polyclonal Antibody, Unconjugated (bs-10406R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Tissue/cell: Rat brain; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Anti-Thyroid peroxidase Polyclonal Antibody, Unconjugated(bs-10406R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining



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