Starting Material （Amount）: Up to 1 g tissue,
Up to 1 x 10^7 cells
Scale: Scaleable
System: TRIzol
Quantity: 1 
Product Size: 100 ml 10296010- TRIZOL LS Reagent 100ml（長方形）Invitrogen *
Final Product: Transcriptome RNA,
micro RNA,
Total RNA
Yield （up to）: 8 mg
Purification Time: 1 hr
Number of Reactions: 100 Preps
Isolation Technology: Organic Extraction
Sample Type （General）: Viral Samples,
Liquid Samples （e.g. Serum）
Downstream Application: Nuclease Protection Assays,
RT-PCR （Endpoint）,
Northern Blotting,
Real-Time PCR,
Cloning,
cDNA Library Construction
High Throughput Compatibility: Manual Protocols,
Not High Throughput-Compatible
TRIzol® LS Reagent is a ready-to-use mono-phasic solution of phenol and guanidine isothiocyanate, designed for the isolation of total RNA from liquid samples of human, animal, plant, yeast, bacterial, and viral origin. During sample homogenization or lysis, TRIzol® LS Reagent maintains the integrity of the RNA, while disrupting cells and dissolving cell components.
Key features of TRIzol® LS Reagent:10296010- TRIZOL LS Reagent 100ml（長方形）Invitrogen *

• Designed for use with liquid samples such as serum and virus preparations
• Uses a TRIzol® LS Reagent:liquid sample ratio of 3:1
• Facilitates isolation of a variety of RNA species of large or small molecular size
Total RNA isolated by TRIzol® LS Reagent is free of protein and DNA contamination. It can be used for Northern blot analysis, dot blot hybridization, poly（A）+ selection, in vitro translation, RNase protection assays, RT-PCR, and molecular cloning. For RT-PCR, treatment of the isolated RNA with amplification grade DNase I （Cat. No. 18068-015） is recommended when the two primers View Morelie within a single exon.
: 34661275 

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