Cat. Number: Rs-0741R

Quantity size: 0.1ml （dilute with pH 7.4 0.01 M PBS or diluent of antibody）

 

Background: This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen （PCNA）, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. Two families of cyclin dependent kinase inhibitors （CKIs） have been identified. The p21WAF1/Cip1 family inhibits all kinases involved in the G1/S transition. The p16INK4a family inhibits Cdk4 and Cdk6 specifically.

 

Anti-WAF1 抗體說明書，報價

Specificity: ·anti-WAF1 is a rabbit monoclonal antibody unconjugated

·specific for WAF1 of hu, rat and mo

·epitope mapping near the amino terminus of WAF1 of

· use for western blotting, elisa, immunoprecipitation

· Protein A affinity chromatography purification, purity :>95%

· Isotype: IgG

· mol wt: 82kDa

 

Application: · Western blotting: 1:100-500

· Immunohistochemistry: 1:100-500

· ELISA: 1:500-1000

· Optimal working dilutions must be determined by the end user.

 

 

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.