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首頁   >>   資料下載   >>   Formation of free and protein-bound carboxymethyllysine and carboxyethyllysine in meats during comme

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Formation of free and protein-bound carboxymethyllysine and carboxyethyllysine in meats during comme

閱讀:427      發(fā)布時間:2018-01-16
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 The effect of commercial sterilization treatments on the levels of advanced glycation endproducts (AGEs) in meats was investigated. The amounts of both free and protein-bound Nε-carboxymethyllysine (CML) and Nε-carboxyethyllysine (CEL) in beef (rump, ribeye, short plate), pork (hind leg, tenderloin, belly), and chicken (chicken breasts, drumsticks) were determined using an HPLC–MS/MS method. Beef and pork had a small proportion (raw b15%; sterilized b8%) of free AGEs compared to the total AGEs, but raw chicken breasts had very high levels of free CEL (7.12 ± 9.98 mg/kg; n = 13) with large biological variation compared to pork (0.19 ± 0.09 mg/kg; n = 9) and beef (0.44 ± 0.19 mg/kg; n = 9). Commercial sterilization (121 °C for 10 min) did not significantly affect the amounts of free CML or CEL, but led to about 0.6- to 3.6-fold increase of protein-bound CML and CEL. The amounts of protein and fat content in beef or pork had very little effect on the formation of protein-bound AGEs during sterilization process.(研究了商業(yè)滅菌法對肉類中晚期糖基化終末產(chǎn)物的影響。利用HPLC-MS/MS方法測定牛肉(臀肉,牛脊肉,胸腹肉)豬肉(后腿,里脊肉)雞肉(雞胸肉,雞腿)中自由基與蛋白結(jié)合CML和CEL含量。豬肉與牛肉有小比例的晚期糖基化終末產(chǎn)物,但是未加工的雞胸肉自由CEL含量高。商業(yè)滅菌(121℃ 10min)沒有對自由CML,CEL產(chǎn)生顯著的影響,但是使蛋白結(jié)合CML和CEL的量增加了0.6-3.6倍。牛肉或豬肉中的蛋白質(zhì)和脂肪含量對在殺菌過程中形成蛋白質(zhì)結(jié)合晚期糖基化終末產(chǎn)物的影響很小。)

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