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| 產品英文名稱 | IPKine™ Anti-GFP Magnetic IP Kit |
| 產品中文名稱 | IPKine™ GFP標簽蛋白免疫沉淀試劑盒（磁珠法） |
| 免疫原 | 合成多肽 |
| 試劑盒組分 | Non-Denaturing Lysis Buffer TBS (10×) Anti-GFP Magnetic Beads Mouse IgG Magnetic Beads Elution Buffer Neutralization Buffer SDS-PAGE Loading Buffer (5×) |
| 特點&優勢 | ? 高效：特異性強、靶蛋白結合量高，≥0.6 mg GFP標簽融合蛋白/mL磁珠； ? 通用：提供IP實驗所需的所有必要緩沖液； ? 可靠：提供陰性對照，可排除IgG本身和目的蛋白或其它特定生物分子的非特異性結合； ? 靈活：本試劑盒提供兩種洗脫方法（酸洗脫和SDS-PAGE Loading Buffer洗脫方法）。 |
| 保存建議 | 按各組分標簽提示分開存儲，保質期12個月。 |
| 運輸條件 | 冰袋運輸（藍冰） |
| 警告 | 本文列出的產品僅供研究使用，不適用于人類或臨床診斷。我們產品所推薦應用，不是建議使用我們的產品去違反任何或許可證。對于使用本產品可能發生的侵權或其他違規行為，我們不承擔任何責任。 |
| 背景 | The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9kD) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish. The GFP has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. |
Figure. The immunoprecipitation effect of Anti-GFP Magnetic IP Kit used for GFP-Tag fusion protein. HEK293T cells were transfected with GFP-Tag plasmid. Lane 1 was whole cell lysate (WCL); Lane 2 was the immunoprecipitation sample of Mouse IgG Magnetic Beads eluted by 1×SDS-PAGE Loading Buffer; Lane 3 was the immunoprecipitation sample of Anti-GFP Magnetic Beads eluted by 1×SDS-PAGE Loading Buffer. Lane 4 was the immunoprecipitation sample of Anti-GFP Magnetic Beads eluted by Elution Buffer. By using acid elution, only contained GFP-Tag fusion protein, did not contain heavy and light chains of antibody.
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