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含 7 個氨基酸的多肽，DOTA偶聯(lián)多肽GRGDSPC
閱讀：248 發(fā)布時間：2022-10-10名稱：DOTA-GRGDSPC，DOTA偶聯(lián)多肽GRGDSPC
一種含 7 個氨基酸的多肽，是一種末端巰基化的細(xì)胞粘附肽。GRGDSPC, a 7-amino acid peptide, is a thiolated cell adhesion peptide.
GRGDSPC通過巰基丙烯酸反應(yīng)與丙烯酸葡聚糖偶聯(lián)，以調(diào)節(jié)人類間充質(zhì)***（hMSCs）與光交聯(lián)水凝膠的相互作用。為了確定GRGDSPC肽與DEX-MAES16的結(jié)合動力學(xué)和效率，在pH 7.8的PBS中，隨著時間的推移（0.25、0.5、1和3h），各種GRGDSPC-濃度（即5、10和20 mg/1 g DEX-MAES 16）與丙烯酸葡聚糖（DEX）大分子單體結(jié)合，并使用Ellman分析法量化未反應(yīng)肽的游離巰基。此外，還比較了巰基肽與丙烯酸酯（DEX-MAES16）和甲基丙烯酸（DEX-HEMA16）大分子單體的反應(yīng)動力學(xué)。早在15分鐘接合時，用5、10和20 mg GRGDSPC肽/1 g修飾的DEX，與DEX-MAES的肽接合效率分別為105.40、94.10和87.45%，而與DEX-HEMA的反應(yīng)分別為0.73、15.78和18.42%。1h后，GRGDSPC與DEX-MAES的接合在肽濃度為10mg時完成，但肽中只有35.66%的巰基與DEX-HEMA反應(yīng)。反應(yīng)動力學(xué)也在接合3小時時進(jìn)行監(jiān)測，所有20 mg GRGDSPC肽與丙烯酸DEX反應(yīng)，而此時甲基丙烯酸DEX僅為32.53%。
GRGDSPC is conjugated to acrylated dextran via thiol-acrylate reaction to regulate the interactions of human mesenchymal stem cells (hMSCs) with the photocrosslinkable hydrogels. To determine the conjugation kinetics and efficiency of GRGDSPC peptide to DEX-MAES16, various GRGDSPC concentrations (i.e., 5, 10 and 20 mg/1 g DEX-MAES16) are conjugated to the acrylated Dextran (DEX) macromer over time (0.25, 0.5, 1 and 3h) in PBS at pH 7.8 and the free thiol groups of unreacted peptides are quantified using Ellman&＃39;s assay. In addition, the reaction kinetics of the thiol-peptide to acrylated (DEX-MAES16) and methacrylated (DEX-HEMA16) macromers are compared. As early as 15 min conjugation, with 5, 10 and 20 mg of GRGDSPC peptide/1 g modified DEX, the peptide conjugation efficiencies with DEX-MAES are 105.40, 94.10 and 87.45%, respectively, while for the reaction with the DEX-HEMA they are 0.73, 15.78 and 18.42%, respectively. After 1h, the GRGDSPC conjugation with DEX-MAES is completed with the peptide concentration of 10 mg, but only 35.66% of the thiol groups of the peptide react with DEX-HEMA. The reaction kinetics are also monitored at 3 h of conjugation, and all of the 20 mg GRGDSPC peptide reacts with acrylated DEX compared to only 32.53% for the methacrylated DEX at this time point.
中文名稱：GRGDSPC
英文名：GRGDSPC
CAS號：91575-26-7
單字母：H2N-GRGDSPC-OH
三字母：H2N-Gly-Arg-Gly-Asp-Ser-Pro-Cys-COOH
氨基酸個數(shù)：7
分子式：C25H42N10O11S1
平均分子量：690.73
***分子量：690.28
等電點(diǎn)(PI)：8.55
pH=7.0時的凈電荷數(shù)：0.94
平均親水性：1.325
疏水性值：-1.24
來源：Synthetic
純度：95%、98%
鹽體系：可選TFA、HAc、HCl或其它
生成周期：2-3周
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