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Source

Rabbit

Applications

IHC,IF,ELISA

Reactivity

Human,Mouse,Rat

Dilution

IHC: 1:100 - 1:300. IF: 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Storage

-20°C/1 year

Specificity

ADD1 Polyclonal Antibody detects endogenous levels of ADD1 protein.

Source/Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen

The antiserum was produced against synthesized peptide derived from human ADD1. AA range:411-460

Uniprot No

P35611

Alternative names

ADD1; ADDA; Alpha-adducin; Erythrocyte adducin subunit alpha

Form

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Background

adducin 1(ADD1) Homo sapiens Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq, Jul 2008],

Other

ADD1, Alpha-adducin

Mol.Wt (Da)

80955

Concentration

1 mg/ml