The multifunctional, multi -compartmental protein Calreticulin (Crt) functions as a soluble molecular chaperone of new or misfolded proteins, as well as a Ca2+-binding protein. Most abundant in the ER lumen, Crt expression also occurs in other membrane-bound organelles, the cell surface, and extracellularly. Also known as CRP-55, calregulin and HACBP (high affinity calcium-binding protein), Crt contains the ER-retrieval sequence, KDEL, and is the solub le paralog of the ER membrane protein Calnexin (Cnx). Crt's three domains include a 180 residue N-terminal domain, a proline-rich Pdomainresidues 189 -288) that binds Ca2+ with high affinity and shares homology with Cnx and calmegin, and a 110 residue C-terminal domain that binds Ca2+ with low affinity but high capacity. The P-domain may interact with the co-chaperone ERp57 (Grp58), a thiol reductase. The NMR structure of the P -domain consists of an extended hairpin that appears to form a curved protrusion from the Crt core domain. Both Crt and its membrane bound homolog CNX interact with proteins and glycoproteins possessing monoglucosylated N -glycans. The Crt/Cnx cycle promotes correct folding, inhibits aggregation of folding intermediates, blocks premature oligomerization, regulates ER degradation, and prevents incompletely folded glycoproteins from exiting to the Golgi complex. Crt also appears to function as an auto-antigen in systemic lupus erythematosus, rheumatoid arthritis, celiac disease, complete congenital heart block, and halothane hepatitis. A diversity of additional functions attributed to Crt includes adhesion, blood function, and cardiac and neuronal development gene expression.

多功能、多區(qū)室蛋白鈣網(wǎng)蛋白（Crt）可作為新的或錯(cuò)誤折疊的蛋白質(zhì)的可溶性分子伴侶，以及Ca2+結(jié)合蛋白。Crt 表達(dá)在 ER 管腔中最為豐富，也發(fā)生在其他膜結(jié)合細(xì)胞器、細(xì)胞表面和細(xì)胞外。Crt 也稱(chēng)為 CRP-55、鈣調(diào)節(jié)蛋白和 HACBP（高親和力鈣結(jié)合蛋白），含有 ER 檢索序列 KDEL，是 ER 膜蛋白鈣連接蛋白 （Cnx） 的可溶物。

CRT 似乎也可作為系統(tǒng)性紅斑狼瘡、類(lèi)風(fēng)濕性關(guān)節(jié)炎、乳糜瀉、完-全性先天性心臟傳導(dǎo)阻滯和氟烷肝炎的自身抗原發(fā)揮作用。Crt 的多種附加功能包括粘附、血液功能以及心臟和神經(jīng)元發(fā)育基因表達(dá)。

艾美捷鈣網(wǎng)蛋白（CRT）單克隆抗體（ADI-SPA-601PE-D）基本參數(shù)：

克隆： FMC 75

宿主： 鼠

同種型： 免疫球蛋白G1

免疫原： 重組鈣網(wǎng)蛋白。

源： 從腹水中凈化

物種反應(yīng)性： 人類(lèi)

猴子

應(yīng)用： 流式細(xì)胞術(shù)

推薦稀釋度/條件： 流式細(xì)胞術(shù) （1：100）

建議的稀釋度/條件可能不適用于所有應(yīng)用。

應(yīng)用說(shuō)明： 預(yù)測(cè)分子量為~63kDa。

純度細(xì)節(jié)： 蛋白G親和純化。

配方： 液。在PBS中，pH 7.2，含有0.09%疊丨氮丨化鈉。

處理： 避免凍融循環(huán)。避光。

航運(yùn)： 藍(lán)冰不結(jié)冰

長(zhǎng)期儲(chǔ)存： +4°C

鈣網(wǎng)蛋白（CRT）單克隆抗體部分文獻(xiàn)引用：

CD47 Blockade Leads to Chemokine-Dependent Monocyte Infiltration and Loss of B Cells from the Splenic Marginal Zone: Y. Y. Yiu, et al.; J. Immunol. 208, 1371 (2022);

IMMUNEPOTENT CRP induces DAMPS release and ROS-dependent autophagosome formation in HeLa and MCF-7 cells: A.C. Martinez-Torres, et al.; BMC Cancer 20, 647 (2020); Full Text

Programmed cell removal by calreticulin in tissue homeostasis and cancer: M. Feng, et al.; Nat. Commun. 9, 3194 (2018);

CD47 Blockade Leads to Chemokine-Dependent Monocyte Infiltration and Loss of B Cells from the Splenic Marginal Zone: Y. Y. Yiu, et al.; J. Immunol. 208, 1371 (2022);

IMMUNEPOTENT CRP induces DAMPS release and ROS-dependent autophagosome formation in HeLa and MCF-7 cells: A.C. Martinez-Torres, et al.; BMC Cancer 20, 647 (2020); Full Text

Programmed cell removal by calreticulin in tissue homeostasis and cancer: M. Feng, et al.; Nat. Commun. 9, 3194 (2018);

鈣網(wǎng)蛋白（CRT）單克隆抗體相關(guān)研究：

鈣網(wǎng)蛋白單克隆抗體 （FMC 75）ADI-SPA-601-F

鈣網(wǎng)蛋白單克隆抗體 （FMC 75）（DyLight™ 488 偶聯(lián)物） ADI-SPA-601-488-F

小鼠 IgG1 同種型對(duì)照，單克隆抗體 （MOPC-21）（PE 偶聯(lián)物） ADI-SAB-600PE-F