3D全自動外泌體熒光檢測分析系統

法國Abbelight公司開發的3D全自動外泌體熒光檢測分析系統是一款無需純化的、全自動的可對單個外泌體進行表征分析的全新設備。該設備能夠提供外泌體表征信息，包括外泌體粒徑大小、亞型分布、攜帶蛋白表達、單個外泌體的膜蛋白與生物標志物共定位等。操作簡單，結果可靠。

3D全自動外泌體熒光檢測分析系統基于特異性免疫捕獲技術，允許研究者直接分析特定群體的外泌體。通過單分子定位技術成像，可以得到單個外泌體的超分辨成像結果，尺度可以到20nm。全自動外泌體熒光檢測分析系統兼容各種生物樣本，除了純化的外泌體之外，對于血液、尿液、惡性腫瘤、腹水中的外泌體也可直接檢測分析，大大拓展了研究范圍。

應用方向及主要特征

超高分辨單外泌體成像

左：2D單個外泌體成像；右：3D單個外泌體成像

外泌體粒徑分析：通過團簇分析外泌體的整體和亞群的粒徑分布

膜蛋白表征與共定位分析，以CD63，CD81&CD9為例表征其單陽，雙陽和三陽的比例

外泌體內容物表征

對比穿膜處理后外泌體中內容物aGFP的表達

觀測細胞中外泌體的分布情況，研究細胞中細胞器或者其他蛋白和外泌體的定位關系

應用案例

法國Abbelight公司開發的3D全自動外泌體熒光檢測分析系統是一款無需純化的、全自動的可對單個外泌體進行表征分析的全新設備。該設備能夠提供單個外泌體表征信息，包括外泌體粒徑大小、亞型分布、攜帶蛋白表達、單個外泌體的膜蛋白與生物標志物共定位等。

應用領域：腫瘤診斷，藥系統開發，眼科疾病診斷，疫苗研發，脊髓受傷機制研究，血漿/血清外泌體分析，外泌體工程化，呼吸疾病診斷

測試數據

外泌體2D成像

外泌體3D成像

外泌體形態分析

發表文章

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